Method Description
Experimental Protocols
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Affymetrix Features
Leu Lab
Murray Lab

Welcome to our web page for mapping the genetic basis of traits.

This page accompanies the paper High-Resolution Mutation Mapping Reveals Parallel Experimental Evolution in Yeast, by Ayellet V. Segrè, Andrew W. Murray and Jun-Yi Leu, PLoS Biology, 2006, 4(8): e256.

Our mapping method can quickly and precisely find the genomic locations of adaptive mutations associated with experimentally and naturally evolved quantitative traits. The method is based on linkage analysis applied to a single pool of segregants that express the trait of interest, also known as Bulk Segregant Analysis, and the genotyping is done using high-density oligonucleotide microarrays. The method was tested in budding yeast, Saccharomyces cerevisiae on five known genes that were mapped to a resolution of 0.2-24 kb (0.1-10 cM), and could be extended to other organisms.

We initially developed this mapping method in the interest of studying the genetic basis of experimentally evolved traits in yeast. In particular, we wish to explore the nature, dynamics and order of occurrence of genetic and phenotypic changes in populations subjected to different selective pressures, and the reproducibility of evolutionary paths between identical, independent experiments.

For comments or questions please contact:

Ayellet Segrè
Laboratory of Andrew Murray
Molecular and Cellular Biology Department, Harvard University
16 Divinity Avenue, Cambridge, MA 02138, USA
asegre [at] broad [dot] mit [dot] edu
Tel: 617-496-1489   -   Fax: 617-496-1541

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Last updated August 6, 2006